Biological Safety Cabinet

Question 1 – What work will you be doing?


A BSC should be selected primarily in accordance with the type of protection needed: product protection; personnel protection against Risk Group 1~ 4 microorganisms; personnel protection against exposure to radionuclides and volatile toxic chemicals; or a combination of these.


Cabinet Selection


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Question 2 – What are the size limits for your cabinet? 


Know what the maximum space allotment is so that you don’t end up with equipment that is too big for your lab, or so small that you can’t work. 


Consider: 

•Where will the cabinet be situated 

•Position of other equipment/doors/traffic flow 

•Ceiling heights • 


Sizes available: approx.  

•3 ft (900mm) 

•4 ft (1200mm)  

•5 ft (1500mm)


Other Considerations:

•Top ten factors/features that influence the decision-making process when buying a biological safety cabinet:

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•Source: https://www.labmanager.com/lab-product/2012/08/biological-safety-cabinets#.XbrIDS2cZBw •

Backup System

Q:How long can the optional backup system of the ULT Freezers last?

A:

The holding time of the backup system depends on the user's supply of CO2/LN2

The consumption rate is 3-5 kg/h. Theoretically, the more abundant the CO2/LN2 supply is, the longer the system will last.

Incubation

Q:What are the advantages of Haier Biomedical's CO2 Incubator temperature control?

A:Based on PID control, Haier Biomedical’s CO2 Incubator is heated on all six sides, the temperature is more uniform;

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Dual PT1000 high-precision sensors are used internally, temperature sensing is more precise; according to actual tests, the uniformity of the 27 test points is all within 0.3℃, and the temperature fluctuation is ±0.1℃.

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Q:What is the superiority of Haier Biomedical’s CO2 Incubator in terms of sensors?

A:Haier Biomedical's CO2 Incubator sensor, based on NDIR measurement principle, using silicon MEMS transmitter, it can achieve zero drift and automatic calibration, the offset is less than 0.3% within two years and the control accuracy reach to ±0.1%. New IR sensor with high temperature resistance of 190℃, not affected by temperature and humidity or pressure changes, effectively solving the problem if the door is often opened, and temperature and other parameters are often the major issues. It can carry out more than 300 dry heat sterilization cycles, with a service life of up to 15 years.


Q:Are thermal conductivity sensor and infrared sensors optional? Can I choose copper liner?

A:The Haier Biomedical CO2 Incubator is equipped with an infrared sensor as standard, and the thermal conductivity sensor is not currently supported; the incubator is manufactured with a 304 stainless steel liner, and the optional copper liner is not currently supported.


Q:What is the status of 100% saturated humidity? What should I do if the humidity is too high?

A:Saturated humidity is a critical state. Humidity can only be infinitely close to but still less than 100%.


High saturation humidity is beneficial to the cultivation of cells, but if the laboratory is not properly disinfected, it will also lead to bacterial growth. If this problem occurs, it is recommended to use a water tray to humidify the bottom, which can effectively reduce the humidity.


Q:Under what conditions will the door ajar alarm be triggered?

A:Haier Biomedical CO2 Incubator will trigger the ajar alarm when the door has been opened for 30 seconds.


Q:How to solve the problem of abnormal liquid level display?

A:The bottom liquid level monitoring has been tested by adding water at the bottom. When the user uses ultra-pure water, the conductivity of the water is relatively high, which results in the liquid level controller being undetectable. The problem can be solved by adding electrolytes such as copper sulfate or saline. 


Q:How long is the overseas warranty period? Is there a sterilization test report?

A:The overseas warranty period is 1 year; and the sterilization report used is the TUV test.


Q:Are there any components in the incubator that need to be removed before the sterilization process?

A:No, none of the standard component of incubator needs to be removed. However, the filters which can’t withstand the high temperature should be taken out


Q:Do infrared sensors never need to be calibrated?

A:Calibration is not required for short term use while for long term use the calibration is needed


Q:What does that mean if the water level displays red?

A:It means the water level isn’t detected.  We don’t recommend the ultra-pure water which will corrode the metal. The Sterilized distilled water is recommended.


Q:What should we do if the sterilization is failed?

A:

Step 1: Check if the water at the bottom is not completely drained, if not, wipe the bottom with a dry cloth or paper towel

Step 2: Restart the machine and confirm whether the position of the liquid level switch is displayed in red. If yes, sterilize the machine


Liquid Nitrogen Storage Solutions

Q:

What is the average liquid nitrogen consumption of YDD series stainless steel liquid nitrogen containers, and what type of liquid nitrogen supplement is used?

A:

Taking YDD-850-465 as an example, the average daily liquid nitrogen consumption is 7.8442 L. It adopts a manual and automatic integrated liquid nitrogen supplement form, users do not need to replace the connecting pipe interface when refilling liquid nitrogen, which is much more convenient and safer than the traditional operation of pouring liquid nitrogen from the opening head of the container.



Q:

How to monitor the status inside the YDD series stainless steel liquid nitrogen container to ensure sample safety?

A:

In terms of temperature and liquid level measurement, Haier Biomedical offers two measurement methods for users to choose: differential pressure and capacitance; temperature and liquid level are measured and controlled separately, with interlocking function to achieve multiple security guarantees; at the same time, multiple alarm signals are in place to ensure sample safety; support external network, internal network, wired and wireless network communications to achieve remote monitoring.


Q:

Can the structure of the YDD series stainless steel liquid nitrogen container achieves gas-phase storage?

A:

Both gas and liquid phases can be used; the lowest temperature at the top of the freezer rack can reach -190°C when it comes to gas phase storage.


Q:

What are the characteristics of the auxiliary access extraction device for the freezer rack?

A:

Our freezing storage rack auxiliary access extraction device has the following five features that are easy for users to operate: adopting slide rail design, the user can freely slide the sling to the lifting position; the lifting system is manouverd by remote control; equipped with weight limit protection function, the weight limit is 20kg; the sling hook has a laser positioning function; the rotation angle is 350°, easy to move in any direction.

 

Q:

What is the working principle of YDZ series self-pressurized supply container?

A:

When the inner container is filled with liquid nitrogen, the vent valve, the discharge valve and the pressure reduction valve is closed, and the liquid nitrogen injection port is screwed tightly. In the case of no leakage, due to the heat transfer of the container shell to the pressure increasing tube, part of the liquid nitrogen that enters the tube by itself is absorbed due to the heat transfer and is vaporized.

 

When the pressure increases valve is turned opened, the vaporized nitrogen passes through the valve and immediately enters the upper space above the inner container liquid surface. At the same time, the liquid nitrogen in the container inner container continuously enters the pressure increase tube to absorb the heat and is vaporized. Due to the volume of vaporized nitrogen being more than 600 times that of liquid nitrogen, a large amount of gas phase nitrogen obtained through a small amount of liquid nitrogen being vaporized continuously enters the inner container through the opened pressure increase valve. With the increase in the amount of entering nitrogen, the nitrogen accumulated in the upper space above the liquid surface of the inner container gradually generates pressure on the inner wall and the liquid surface. When the pressure gauge value reaches 0.02 MPa, open the discharge valve and the liquid nitrogen will be discharged through the drainage pipe.


Q:

What are the features of YDZ series self-pressurized supply containers?

A:

Convenient to fill liquid nitrogen: just unscrew the plug of the liquid nitrogen injection port, you can use the liquid machine fractionation tower discharge outlet or pipeline to fill.

 

Fast pressurization and convenient operation: The pressurization method of applying pressure generated after a small amount of liquid nitrogen is vaporized to the inner container, which is convenient for operation, fast to raise pressure, and ensures the purity of liquid nitrogen.

 

Superior safety and reliability: The discharge pressure of the container is extremely low, and it is equipped with a pressure gauge, a safety valve, and a vacuum sealed joint. Therefore, the container is safe and highly reliable in use.

 

High strength materials: The outer shell, inner container and main parts are all made of austenitic stainless steel with good vacuum and low temperature performance.


Q:

What settings are the YDS series aluminum alloy liquid nitrogen containers suitable for?

A:

It can be used in biobank, laboratory sample storage, sample transportation and other settings. The product volume covers 2-175L which can meet different storage capacity requirements and space conditions. Also, the transportation series can meet the transportation needs and can be equipped with smart cap. The temperature data during transportation is clear at a glance; it adopts gas phase storage, no fear of liquid nitrogen overflow; stainless steel mesh screen divides the storage which makes it easier to store different samples.


Q:

What technologies are used in YDS series aluminum alloy liquid nitrogen containers?

A:

The inner container insulation material is handled by automatic winding equipment; adopting the intermittent evacuation process, the evacuation period is longer than 120 hours; the product surface is sprayed with plastics; all products are tested at 100% liquid nitrogen, and the liquid nitrogen loss of each product is accurately recorded to verify the reliability of each product under low temperature conditions.

Transport Cooler
Q:What is the current transport cooler categories and range available from Haier Biomedical?

A:

At present, we mainly have active cooling series and passive cooling series, with storage capacity ranging from 5-35L.

Meet different temperature requirements of 2-8℃, -15℃~-25℃, -80℃ etc.


Q:What are the main components of the transport cooler?

A:Haier Biomedical transport cooler consists of 3 parts, from inside out: main container, auxiliary container and outer packaging.


The highly pathogenic microorganism species or samples shall be safely stored in the main container, which shall be sterile, impermeable and leakproof. The main container is made of either glass, metal or plastic and is manufactured with a reliable leak-proof seal, such as heat seal, flange stopper or metal coiled-edge seal. The main container is wrapped with enough sample absorbent material to absorb all the samples in case of leakage. It meets the P620 packaging requirements for Class A infectious substances, and can be used to transport UN2814, UN2900, and UN3373 infectious substances;


The auxiliary container is a strong, waterproof and leak-proof container which is beside the main container. Its function is to package and protect the main container. When multiple primary containers are loaded into an auxiliary container, they must be wrapped separately to prevent contact with each other and lined with adequate absorption material, if a leakage occurs, all samples will be absorbed. 


The main container and auxiliary container can withstand 95kPa of internal pressure without leakage and is able to withstand damage at temperatures ranging from -40℃ to +55℃; The main and auxiliary containers can be kept intact with the temperature at which the refrigerant is used and at the temperature and pressure that may occur after the loss of refrigeration.


The outer packaging is a protective layer on the outer surface of the auxiliary container. The outer packing has sufficient strength and shall be affixed with uniform marks on the outer surface as required.


Q:How does the Haier Biomedical transport cooler protect the safety of samples?


A:Haier Biomedical's active transport cooler can ensure accurate temperature control of 2-6°C, and the temperature display accuracy reaches to 0.1°C; the passive cooling transport cooler has a long insulation time, which can be maintained between 2-10°C for 8 hours; in terms of safety assurance, all models have an authorized unlocking function, and are also equipped with alarm functions such as temperature alarm and battery alarm, providing multiple guarantees for sample security and safety.

ULT Freezer

Q:How to choose a medical refrigerator? 

A:

Temperature

8~20℃

2~8℃

-25℃

-30~-40℃

≤-60℃

-80℃

≤-150℃

Item classification

Medicine

Reagent

Specimen

Culture medium

Medicine

Vaccine

Reagent

Red blood cell (2~6℃)

Poliomyelitis vaccine

Enzyme

Protein

Plasma

Pharmaceutical ingredients

Pharmaceutical ingredients

Tuna

High-end food ingredients

Blood

Urine

Serum

Blood clot

Liquid samples stored for future RNA   and DNA extraction

Frozen red blood cell

Samples with unidentified future use

Cell samples

Recommended product

Pharmaceutical cool refrigerator

Pharmaceutical refrigerator

-25℃ series

-30/-40℃ series

-60℃ series

-86℃ series

-150℃ series

Liquid nitrogen container


Q:Is stainless steel tank optional for ULT freezers?

A:Stainless steel tank is optional for all ULT models.


Q:Is the printer optional for ULT freezers?

A:A printer is optional for all ULT models. However, it is not recommended to actively promote it to the users.


Q:Do ULT freezers have an adjustable tray? 

A:An adjustable tray is available as an accessory, used to place samples and other items when accessing the freezer for user convenience.


 Q:Do ULT freezers have a factory inspection report?

 A:Each freezer comes with a test report when leaving the factory.


Q:What are HC Refrigerants? How are refrigerants classified?

A:Depending on the extent to which freon destroys the atmospheric ozone layer, the refrigerants are generally classified into CFC, HCFC, HFC, and HC.

● CFC- Chlorofluorocarbon. It has been banned worldwide as a public nuisance. It contains chlorine and no hydrogen and damages the ozone layer seriously;

For example: R11 (fluor trichloromethane), R12 (difluoro dichloromethane), R13 (Trichlorofluoromethane), and R14 (carbon tetrafluoride), etc.


● HCFC- Hydrochlorofluorocarbon. It has been banned in developed countries and China as a transitional substance with low public nuisance. It contains chlorine and hydrogen.

For example: R22 (difluorochloromethane), R123 (Dichlorotrifluoroethane), R124 (Chlorotetrafluoroethane), and R142b (Chlorodifluoroethane), etc.


● HFC- hydrofluorocarbon. It has been officially banned by developed countries, such as the EU and Australia, in 2020. It is chlorine-free and pollution-free and can be used as an alternative refrigerant, but it contributes significantly to the greenhouse effect.

For example: R134a (tetrafluoromethane), R125 (pentafluoro ethane), R32 (difluoromethane), R23 (trifluoro methane), R116 (hexafluoroethane), R143a (trifluoroethane), R404A (a mixed refrigerant made up of 44% R125 + 4% R134A + 52% 143A), R508B (a mixed refrigerant made up of 46% R23 + 54% R116), R410A (a mixed refrigerant made up of 50% R32 + 50% R125), and R245fa (perfluoro propane), etc.


● HC - hydrocarbon. It is widely used and promoted by the EU, the US, Australia, and China as a pollution-free product of nature. It is chlorine-free and fluoride-free. As an alternative refrigerant, it contributes little (almost 0) to the greenhouse effect.

For example: R600a (iso-butane), R290 (propane), R170 (ethane), R1150 (ethylene), and R50 (methane), etc.


Q:How can an ULT Freezer with a large volume enter through a door opening?

A:

● For ULT freezers of 728/828/959L and above, the minimum door size shall be 890mm. 

● The DW-86L578 can pass through an 830mm door after removing the handle. 

● The DW-86L338J/388J can pass through a 750mm door.


Q:How much does an ULT Freezer weigh when it is fully loaded?

A:Generally, it is the net weight of the freezer plus the weight of the fully-loaded samples:

The weight of a 5*5 cryogenic rack is considered to be 17kg:

The weight calculation of the freezer shall refer to the number of cryogenic racks installed for each model as shown in the product catalogue:


Q:How to query the official website screenshot of the Energy Star Certification?

A:Website: https://www.energystar.gov/products/spec?s=mega


Q:What are the differences between the various external interfaces?

A:

● About RS232 interface:

It is used to monitor the running state of the freezer remotely. It sends digital signals within a transmission distance of 20 meters and allows one-to-one communication only. It has been gradually replaced by RS485.


● About RS485 interface

It is used to monitor the running state of the freezer remotely. It sends digital signals within a transmission distance of up to one thousand meters and allows one-to-many communication. Haier makes this kind of interface available.


● About 4-20mA interface:

It can only be used to monitor the freezer temperature via analog signals. It is mostly used in the early old models of Thermo.


● About Dry contacts interface:

It can only be used to provide a switching signal for alarm, achieving a connected or disconnected state in case of any alarm. Haier makes this kind of interface available.


● About Ethernet interface

It is used to monitor the running state of the freezer remotely via digital signal and is mostly used in PHCBI.


Q:How to replace the battery for an ULT Freezer?

A:The batteries of our ULT freezers all provide a 12V power supply:

● The BP/T, S/ST series uses lithium batteries. 

● Other series use lead-acid batteries.


Q:How can an ULT Freezer be fixed in a specific position?

A:In addition to 4 universal casters, each ULT freezer is provided with 2 fixed feet.


Q:What  is the difference between the IoT module and the cold chain module of ULT Freezers?

A:

● IoT module (blue): It must be used together with the ULT freezers. As it has no independent temperature probe, it cannot work alone. 

● Cold chain module (white): It has an independent temperature probe. It can be used together with the ULT freezers and monitor all refrigerators including the rivals’ refrigerators.


Q:What's the warranty of ULT and Biomedical Freezers?

A:3 years for the complete machine and 5 years for the compressor

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