Biosafety Cabinets: Tips for Best Practices

Air extraction

The class of BSC can be further characterised by whether air is recirculated or ducted out of the building. The option that you choose will depend on the severity of risk to the environment and laboratory personnel from the contaminated air according to the BSL. It is important to remember that HEPA filters only extract particles, they do not remove vapours or gases, so these will not be filtered out of the air.

Air flow monitoring

It is beneficial to select a BSC that alerts you if the airflow falls below acceptable levels. 

Certifications and sizing

Ensure that the BSC meets relevant international standards and certifications, such as NSF/ANSI 49 in the US or EN 12469 in Europe.

Sizing

As with any piece of laboratory equipment, the size of a BSC should be an important consideration to ensure that you strike the right balance between providing sufficient working space and optimising valuable lab space. BSCs can vary massively in size, from a couple of metres for multiple users to smaller single-user designs. Manufacturers may even build bespoke cabinets, depending on quantity, sizing requirements and capabilities. 

The aperture

There are also various types of front aperture openings that may vary on the class of BSC or the style. For example, class I BSCs often have a sash opening, while class III have heavy-duty rubber glove apertures to protect the operator. Openings may be manually or electronically controlled, depending on the user’s preference.

Ease of cleaning and decontamination

BSCs should have features that enable easy cleaning and decontamination, such as removable grills and easy-to-reach surfaces. Careful consideration must be given to where removable features – such as night doors – are stored while not in use, to prevent contamination on these surfaces. 

Ergonomics

Researchers can work in a BSC for extended periods of times, so regard should be given to their comfort to avoid injury or overexertion. BSCs with arm rests, extended access openings and sloped viewing windows or side windows can offer enhanced ergonomics.  

Additional features

There are various other features to consider – such as the number of power sockets – that can improve the ease of work. Custom-built instruments can be designed to your specific requirements, but are often more expensive and less adaptable to different applications. 

Service and support

Work with a reputable manufacturer that provides installation and training, and excels in customer support, warranties and service contracts.